DASbox® Mini Series Bioreactor Systems from Eppendorf

(1) Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic mediaMicrobial Cell FactoriesDecember 1, 2022J. Carl Schultz, Shekhar Mishra, Emily Gaither, Andrea Mejia, Hoang Dinh, Costas Maranas, Huimin ZhaoDGA1Δ-ACC1-ACL1) and LRO1Δ-based strains (LRO1Δ, LRO1Δ-ACL1, and LRO1Δ-ACC1-ACL1) were tested in 250 mL Eppendorf DASbox Mini Bioreactors with a 20% dodecane overlay in week-long fed-batch fermentations with glucose concentrations

(2) Integrated continuous biomanufacturing on pilot scale for acidâ€sensitive monoclonal antibodiesBiotechnology and BioengineeringAugust 1, 2022Hubert Schwarz, JoaquÃn Gomis Gomisâ€Fons, MadelÃ¨ne Isaksson, Julia Scheffel, Niklas Andersson, Andreas Andersson, Andreas Castan, Anita Solbrand, Sophia Hober, Bernt Nilsson, et al.small scale, the seed and production bioreactors were identical systems, consisting of a stirred tank DASbox Mini Bioreactor system (Eppendorf) coupled with an alternating TFF system ATF2 (Repligen) with hollow fiber

(3) Combination of Genome-Scale Models and Bioreactor Dynamics to Optimize the Production of Commodity ChemicalsFrontiers in Molecular BiosciencesApril 27, 2022Jorge LÃ¡zaro, Giorgio Jansen, Yiheng Yang, Mario A. Torres-Acosta, Gary Lye, Stephen G. Oliver, Jorge JÃºlvezthe maximization of λ[t cout ]. 2.5.3 Fermentation Experiments Continuous cultures were grown in a DASbox® Mini Bioreactor System (Eppendorf, Stevenage, UK). The E. coli strain used for all fermentation experiments

(4) Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactorsNature protocolsFebruary 1, 2022Ackermann M, Rafiei Hashtchin A, Manstein F, Carvalho Oliveira M, Kempf H, Zweigerdt R, Lachmann N.75004371) DASbox Mini bioreactor system for cell culture applications (Eppendorf, cat. no. 76DX04CC) DASbox Mini bioreactor vessel for cell culture applications (Eppendorf, cat. no. 76DS0250ODSS) DASware® control

(5) Efficient production of immunologically active Shigella invasion plasmid antigens IpaB and IpaH using a cell-free expression systemApplied Microbiology and BiotechnologyJanuary 1, 2022Neeraj Kapoor, Esther Ndungo, Lucy Pill, Girmay Desalegn, Aym Berges, Edwin V. Oaks, Jeff Fairman, Marcela F. PasettiFor exogenous addition of untagged IpgC during synthesis, IpaB was expressed at large scale using a DASbox mini bioreactor system (Eppendorf, Enfield, CT) with 3 μg/ml of plasmid DNA added to the reaction. Expression

(6) Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactorsSTAR protocolsDecember 17, 2021Manstein F, Ullmann K, Triebert W, Zweigerdt R.225,Hamilton,Cat#237452 DASbox Mini bioreactor system for cell culture applications,Eppendorf,Cat#76DX04CC DASbox Mini bioreactor vessel for cell culture applications,Eppendorf,Cat#76DS0250ODSS DASbox exhaust system,Eppendorf,Cat#76DXOFF "DASbox

(7) Site-specific antigen-adjuvant conjugation using cell-free protein synthesis enhances antigen presentation and CD8+ T-cell responseScientific ReportsDecember 1, 2021Adam M. Weiss, Jainu Ajit, Tyler J. Albin, Neeraj Kapoor, Shilpa Maroju, Aym Berges, Lucy Pill, Jeff Fairman, Aaron P. Esser-Kahnsoluble fractions of each protein. For large scale expression of OVA [K20/K370-pAMF]-TEV-his6, the DASbox Mini Bioreactor System (Eppendorf AG) was used. Expression was performed at 25 °C and pH 7.2 for 10 h with

(8) Transition from static culture to stirred tank bioreactor for the allogeneic production of therapeutic discogenic cell spheresStem Cell Research & TherapyDecember 1, 2021Daniel Rodriguez-Granrose et al.fifth modality utilized active control, cells were grown in 0.25 L glass stirred tank bioreactor (0.25L DASbox Mini Reactor Systems, Eppendorf). STR vessels were siliconized with Sigmacote prior to use in order to mitigate

(9) Critical Analysis of cGMP Large-Scale Expansion Process in Bioreactors of Human Induced Pluripotent Stem Cells in the Framework of Quality by DesignBioDrugsNovember 2, 2021Araceli Rivera-Ordaz, Valeria Peli, Paolo Manzini, Mario Barilani, Lorenza Lazzaribar,E8,RB,50,10,"Microcarriers (SoloHill microcarriers, Pall)",3–7 × 104 cells/cm2,,1.4 × 106,,3.5,[126] DASbox Mini BioreactorSystem (Eppendorf),Pitched-blade impeller,mTeSR1; E8,RB; P,125,7,Aggregates,5 × 105 cells/mL,21,3

(10) Physiological Characterization of Sulfolobus acidocaldarius in a Controlled Bioreactor EnvironmentInternational Journal of Environmental Research and Public HealthMay 21, 2021Kerstin RastÃ¤dter, David Johannes Wurm, Oliver Spadiut, Julian Quehenbergerof Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany), was continuously cultivated in a DASbox Mini Bioreactor System (Eppendorf AG, Hamburg, Germany) with a working volume of 200 mL per vessel. Four

(11) Selection for CD26− and CD49A+ Cells From Pluripotent Stem Cells-Derived Islet-Like Clusters Improves Therapeutic Activity in Diabetic MiceFront Endocrinol (Lausanne)May 5, 2021Kfir Molakandov et al.S1 and S2 ). For Bioreactors, the hESC aggregation and differentiation was similarly done in the DASbox® mini system (Eppendorf) with online monitoring of culture parameters. Up to four parallel bottles containing

(12) Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular VesiclesInternational journal of molecular sciencesApril 9, 2021Andrade AC, Wolf M, Binder HM, Gomes FG, Manstein F, Ebner-Peking P, Poupardin R, Zweigerdt R, Schallmoser K, Strunk D.conducted as published [30]. In short, a single cell suspension of human iPSCs was inoculated in the DASbox Mini Bioreactor System (Eppendorf, Hamburg, Gemany) in E8 medium supplemented with Y-27632 at a cell density

(13) Optimisation of Recombinant Myrosinase Production in Pichia pastorisInternational Journal of Molecular SciencesApril 1, 2021Zuzana Rosenbergová, Kristína Kántorová, Martin Šimkovič, Albert Breier, Martin Rebrošfermenter has never been reported. Therefore, high-cell density process in 350 mL mini fermenters (DASbox® Mini Reactor Systems, Eppendorf, Germany) with a 150 mL working volume was optimised. In the glycerol-growth

(14) High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modelingStem Cells Transl MedMarch 4, 2021Felix Manstein et al.Germany, https://www.eppendorf.com) for parallel operation of four or more independently controllable DASbox Mini bioreactor vessels for cell culture applications equipped with an eight‐blade impeller (60° pitch) optimized

(15) An overview of drive systems and sealing types in stirred bioreactors used in biotechnological processesApplied Microbiology and BiotechnologyMarch 1, 2021Cedric Schirmer, Rüdiger W. Maschke, Ralf Pörtner, Dieter Eibl320,18,+,-,od),+,+,+,-,o,+ Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf,Eppendorf BioBlu c,40,+,-,+,-,-,+,-,+,- BioBlu f,3.75,+,-,+,-,+,-,-,+,- DASbox Mini BR,0.25,+,-,-,+,+,+,-,-,+ DASGIP Bioblock,1.8,+,-,-,+,+,+,-,-,+ DASGIP Benchtop,3.8,+,-,-,+,+,+,-,-,+ BioFlo,10.5,+,-,-,+,+,+,+,o,- CelliGen

(16) Microbial upgrading of acetate into 2,3-butanediol and acetoin by E. coli WBiotechnology for BiofuelsDecember 1, 2020Katharina Novak, Regina Kutscha, Stefan PflÃ¼glmeasurements. Cultivations in bioreactors Bioreactor cultivations were carried out in duplicates in a DASbox® Mini Bioreactor system (Eppendorf AG, Hamburg, Germany) at a working volume of 200 ml and a temperature of

(17) Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw materialJournal of Industrial Microbiology and BiotechnologyDecember 1, 2020Katharina Novak, Juliane Baar, Philipp Freitag, Stefan PflÃ¼glhttps://academic.oup.com/jimb/article/47/12/1117/6046071 by guest on 15 November 2021 Bioreactor cultivations were performed in duplicate in a DASbox® Mini Bioreactor system (Eppendorf AG, Hamburg, Germany). The working volume was 200 ml and all cultivations

(18) Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase BScientific ReportsDecember 1, 2020Chrispian W. Theron, Marie Vandermies, Samuel Telek, Sebastien Steels, Patrick Fickersincubation at 30 °C and 150 rpm. Bioreactor cultures were performed for 72 h in duplicate in DASGIP DASbox Mini Bioreactors SR0250ODLS (Eppendorf, Hamburg, Germany). Temperature was set at 30 °C, agitation at 800

(19) Cell-Based Therapy Manufacturing in Stirred Suspension Bioreactor: Thoughts for cGMP ComplianceFrontiers in Bioengineering and BiotechnologyNovember 26, 2020Suman C. Nath, Lane Harper, Derrick E. Rancourt2010 hESC 6 360 28 55 Slow-turning lateral vessel (Synthecon) Gerecht-Nir et al., 2004 hiPSC 5 36 7 125 DASbox mini bioreactor system (Eppendorf AG, Hamburg, Germany) Kropp et al., 2016 hiPSC 2 12 7 100 Spinner

(20) A tunable l-arabinose-inducible expression plasmid for the acetic acid bacterium Gluconobacter oxydansApplied Microbiology and BiotechnologyNovember 1, 2020Philipp Moritz Fricke, Tobias Link, Jochem Gätgens, Christiane Sonntag, Maike Otto, Michael Bott, Tino Polenvisualize FACS data. Bioreactor cultivation of G. oxydans Bioreactor cultivations were conducted in DASbox® mini-bioreactors controlled by DASware software (Eppendorf). In 385-mL glass vessels equipped with two 6-bladed

Be the First to Write a Review!

DASbox® Mini Series Bioreactor Systems

DASbox® Mini Series Bioreactor Systems from Eppendorf

Description

Eppendorf

Eppendorf

Citations (40)

Reviews

DASbox® Mini Series Bioreactor Systems from Eppendorf

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map